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1 Introduction Poor management of plastic waste has resulted in approximately 2 million tonnes of plastic debris entering aquatic environments annually ( The physical characteristics of MPs, particularly their size and colour, closely resemble natural food sources for several marine organisms, leading to active ingestion or accidental capture by species such as planktonic organisms ( Previous studies on adult fish have shown that MPs ingestion can be species-specific, and the rate is influenced by different feeding behaviours and strategies ( The Douro estuary is a nursery for various fish species and exhibits significant MPs contamination ( Pomatoschistus microps , and the sardine, Sardina pilchardus ), which have different life cycles and anatomical features, were used to address fundamental questions about MP bioaccumulation in fish larvae, including when the contamination starts to occur and to provide the MP contamination profile throughout the ontogenetic development stages and in different species

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