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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat GDF5
Spon2 present in the sample is added and binds to antibodies coated on the wells
And then biotinylated Human SMAD4 Antibody is added and binds to SMAD4 in the sample
Only those wells that contain Human VCAN
it binds with Erα bound to precoated antibody
Human TIEG1 (TGF Beta Inducible Early Response Gene 1) ELISA Kit 21-Trihydroxypregn-4-ene-3 Standards or samples are addedThe Human TIEG1 (TGF Beta Inducible Early Response Gene 1) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Human TIEG1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TIEG1 and Avidin Horseradish Peroxidase (HRP) conjugate are added
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